Certificate Course: Blastocyst Biopsy

Course Description:

This specialized course focuses on advanced training in blastocyst biopsy techniques for Preimplantation Genetic Testing (PGT). The hands-on sessions provide comprehensive experience in trophectoderm biopsy and cell preparation for genetic testing using mouse models. Participants will practice preparing biopsy and embryo culture dishes, setting up microscopes and lasers, and performing biopsies on blastocysts at various stages using techniques like "pulling" and "flicking." The program also covers preparation of biopsied cells for genetic analysis, validating cell numbers, vitrification and warming of hatched blastocysts, and assisted hatching (AH) using lasers. Each day includes dedicated half-day biopsy practice sessions to ensure extensive hands-on exposure. Quality assurance, troubleshooting, and best practices are integral components of the training.

Course Objectives:

• Master advanced techniques of blastocyst biopsy.
• Understand embryological and genetic aspects of biopsied blastocysts.
• Learn ethical and legal considerations in PGT.

Prospective Learners:

• Clinical embryologists, geneticists, lab technicians, and researchers in reproductive biology.

Instructional Objectives and Projected Benefits:

• Develop proficiency in advanced biopsy techniques.
• Understand the implications and interpretation of biopsy results.
• Ensure the safety and viability of biopsied blastocysts.

Prerequisite Knowledge and Skills:

• Basic embryology knowledge; prior experience in embryology lab techniques is beneficial.

Training/Educational Methods:

• Theoretical lectures
• Practical demonstrations
• Extensive hands-on training

Certificate of Course Completion:

Participants will receive a certificate upon successful completion and assessment.

Course Structure:

• Day 1:
  • Morning: Introduction to biopsy principles and PGT
  • Afternoon: Biopsy practice session using animal-origin blastocysts
• Day 2:
  • Morning: Preparation of biopsy dishes, assisted hatching, and equipment setup
  • Afternoon: Biopsy practice session using "pulling" and "flicking" techniques
• Day 3:
  • Morning: Preparing biopsied cells for genetic analysis and validation
  • Afternoon: Biopsy practice session with emphasis on cell preparation and vitrification
• Day 4:
  • Morning: Quality assurance, troubleshooting, and procedural recording
  • Afternoon: Final biopsy practice session and practical assessment

Additional Information:

Accommodation can be arranged at the IIT hostel upon request with an extra cost.
Plenty of blastocysts (animal origin) will be provided daily for biopsy practice, ensuring robust hands-on learning opportunities.